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May 2020 – “Pao Pereira Extract Suppresses Benign Prostatic Hyperplasia by Inhibiting Inflammation-Associated NFκB Signaling”

by Yu Dong, Jiakuan Liu, Zesheng Xue, Jingya Sun, Zhengnan Huang, Yifeng Jing, Bangmin Han, Bing Shen, Jun Yan and Ruimin Huang
BMC Complement Med Ther 20, 150 (2020).


Pao pereira extract reduces inflammation in BPH by inhibiting NFKappaB signaling.

Pao pereira (Pau pereira) is incredibly effective for chronic inflammation of the prostate commonly known as BPH or Benign Prostatic Hyperplasia. BPH is an enlargement of the prostate caused by inflammation that is common among older men. The enlarged prostate gives rise to serious urinary problems and quality of life issues.   

A new study from Dr. Jun Yan’s group at Nanjing University in China shows that Pao pereira (Pau pereira) inhibits the control center that is activated in prostate inflammation. In BPH, this master switch, called NFKappaB, is turned on which triggers expression of pro-inflammatory cytokines and genes involved in extracellular matrix remodeling. These cytokines trigger proliferation of prostate cells and further enlargement of the gland. The Pao pereira (Pau pereira) extract turns the switch off so NFKappaB signaling is quieted and the levels of cytokines and remodelers are diminished.

Not only does the extract resolve the inflammatory response in BPH it also reduces viability of the two major cell types in the prostate: epithelial and stromal cells. The chronic inflammation associated with BPH damages and destabilizes the DNA in the cells which renders them susceptible to apoptosis induced by the Pao pereira (Pau pereira) extract.  Apoptosis, or programmed cell death, is triggered in both epithelial and stromal cells in the BPH tissue.

Induction of apoptosis is the precise mechanism of action of the Pao extract in cancer cells which, as Dr. Beljanksi showed, contain destabilized DNA. Malins’ work revealed that cells in the BPH prostate suffer similar DNA damage even though the cells are not cancerous, but rather chronically inflamed or precancerous. The new study from Dr. Yan’s group shows that DNA damage in BPH is sufficient to result in apoptosis in the presence of the Pao pereira (Pau pereira) extract.

There is more!

A previous paper from the Nanjing group showed that Pao pereira (Pau pereira) addresses the androgen imbalance that promotes BPH as men age (read the previous study from the Nanjing group here). The extract inhibits the enzyme that converts testosterone to dihydrotestosterone which triggers prostate enlargement. The Pao extract reduces the size of BPH prostates, lowers the levels of androgen receptor, and lowers PSA–the well-known marker for prostate inflammation. The Pao extract does all of this just as well Finasteride, but without the negative side effects associated with this drug.

Pao pereira (Pau pereira) is indeed incredibly effective for BPH.


BPH Publication May 2020


Our previous study revealed the extract from the bark of an Amazonian tree Pao pereira (Pau pereira) can suppress benign prostatic hyperplasia (BPH) in a rat model. Herein, we examined its inhibitory effects on human BPH cells and dissect its molecular mechanism.


We applied the Pao extract to human BPH epithelial BPH-1 and prostate myofibroblast WPMY-1 cells. Cell viability, apoptosis and immunoblotting were performed, followed by gene expression profiling and gene set enrichment analysis (GSEA) to detect the differentially expressed genes and signaling pathway induced by Pao extract. Human ex vivo BPH explant organ culture was also used to examine the effects of Pao extract on human BPH tissues.


Pao extract treatment inhibited viability and induced apoptosis in human BPH-1 and WPMY-1 cells. Gene expression profiling and the following validation indicated that the expression levels of pro-apoptotic genes (eg. PCDC4, CHOP and FBXO32) were induced by Pao extract in both two cell lines. GSEA further revealed that Pao extract treatment was negatively associated with the activation of NFκB signaling. Pao extract suppressed the transcriptional activity of NFκB and down-regulated its target genes involved in inflammation (CXCL5, CXCL6 and CXCL12) and extracellular matrix (ECM) remodeling (HAS2, TNC and MMP13) in both cultured cells and human ex vivo BPH explants.


In both BPH epithelial and stromal cells, Pao extract induces apoptosis by upregulating the pro-apoptotic genes and inhibiting the inflammation-associated NFκB signaling via reducing phosphorylation of NFκB subunit RelA. Our data suggest that Pao extract may be a promising phytotherapeutic agent for BPH.

Research Publication Data

BPH Publication Fig 1

Figure 1 shows Pao pereira (Pau pereira) inhibited the proliferation and induced apoptosis in BPH-1 and WPMY-1 cells. Image a shows changes of BPH-1 and WPMY-1 cells upon Pao extract treated for 48 h. Image b shows the effects of Pao pereira (Pau pereira) on cell viability by the SRB assay. 

BPH Publication Fig 2

Figure 2 shows Pao pereira (Pau pereira) induced apoptosis in BPH-1 and WPMY-1 cells. Image a shows the flow cytometry analysis on BPH-1 and WPMY-1 cells treated with Pao pereira (Pau pereira) using an Annexin V-FITC/PI double staining kit. Image b shows the cell death rates in BPH-1 and WPMY-1 cells treated with Pao pereira (Pau pereira). Annexin V-positive cells were defined as apoptotic cells. Image c is the effects of Pao pereira (Pau pereira) on the apoptosis-related proteins by Western blotting assay. Data are presented as mean ± SD; * p < 0.05, ** p < 0.01, and *** p < 0.001

BPH Publication

Figure 3 shows Pao pereira (Pau pereira) inhibited NFκB signaling pathway in human BPH tissues. Image a is a schematic diagram of the ex vivo BPH explant culture process. BPH explants were treated with 0, 250, 500 μg/ml Pao pereira (Pau pereira) for 48 h. Image b is the phosphorylation of RelA and protein expression levels of pro-apoptotic and inflammation-associated genes survival-associated proteins (PDCD4 and DDIT3/CHOP) were analyzed by Western blotting in BPH explants with Pao pereira (Pau pereira) treatment. Image c is the mRNA expression levels of target genes from NFκB signaling pathway, pro-apoptotic and inflammation-associated genes in BPH explants with Pao pereira (Pau pereira) treatment by qRT-PCR. * p < 0.05, ** p < 0.01, *** p < 0.001, and ns p ≥ 0.05

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    Cancer Research: Publication on Superior Anti-Cancer Effect of a New Blend of Green Teas

    November 2019 – “Effects of Different Tea Products on the Growth of Cancer Cells”

    Ping Chen and Qi Chen
    EC Nutrition 14.12 (2019): 01-13.

    Kansas university

    Breakthrough Cancer Research by The University of Kansas Medical Center & The Beljanski Foundation

    A recently published study by The University of Kansas Medical Center analyzing the anti-cancer effects of several well-known teas has shown that OnkoTea®, a newly developed blend of green teas, is most effective at inhibiting the growth of multiple cancer cell lines. This blend combines four different green teas: Ceylon Green, Bi Lou Chun, Organic Gunpowder and Dragonwell.

    The anti-cancer potential of each of these four green teas was originally identified by Prof. M. Beljanski of the Pasteur Institute using the Oncotest, an assay he developed for detecting carcinogenic and anti-carcinogenic compounds. The anti-cancer activity of OnkoTea® was compared with the activity of several popular brands of tea including Bigelow, Kusmi and Lipton. The first tests analyzed the activity of OnkoTea® against breast cancer cells including one highly metastatic cell line. OnkoTea® was the best at reducing the number of breast cancer cells.

    OnkoTea® also proved to be effective against liver and bladder cancer cells and showed impressive activity against four melanoma skin cancer cell lines.  The data shows the viability of the cancer cell lines after exposure to extracts of each of the teas that were tested with OnkoTea® “showing the best inhibitory effect.”

    The studies were conducted in vitro and tested a range of concentrations of the teas (200, 400, 600 and 800 micrograms per milliliter) at 24, 48 and 72 hour time points as shown in the images below. Cancer cell lines tested: Breast MCF-7, Metastatic Breast MDA-MB-231; Liver HepG2, Bladder 5637, Melanoma A375, UACC-257, SK-MEL-2, SK-MEL-5.

    Green Tea Anti-Cancer Research Chart

    Green Tea Anti-Cancer Research Chart

    Figures: IC50 values of the OnkoTea®, Lipton, Bigelow & Kusmi tea preparations against melanoma cells. The IC50 values (µg/mL) were derived from the growth curve, defined as the concentration required to inhibit 50% of the cell viability

    Green teas are known to have anti-cancer benefits, but the new cancer research shows that the activity of OnkoTea® stands out for having the most potent anti-cancer effect.

    Current models of carcinogenesis—how cancer starts—tell us that a gradual, but persistent exposure to carcinogens in our environment is responsible. Many of these carcinogens get into our cells, bind to our DNA and damage DNA structure. After chronic interactions with carcinogens the DNA is destabilized—no longer structurally healthy, and the two strands of the duplex are sporadically separated, making the cell amenable to the onset of cancer.

    But if cancer starts by gradual buildup of DNA destabilization, might gradual consumption of a mix of green teas with relatively potent anti-cancer effect be part of the antidote? It takes years for the DNA damage associated with cancer to develop and the habitual consumption of a tea with documented anti-cancer effect can be part of a prevention strategy.


    Green Tea Anti-Cancer ResearchTea preparations are popular for its anti-oxidant and anti-cancer effects. We want to understand how different tea products could differently affect the growth of cancer cells with heterogeneity. Here we evaluated 4 popular tea products on the growth of multiple cancer cells with different genetic makeovers, including 3 green teas and a black tea, namely Bigelow Tea – Green Tea with Mint (Bigelow), Kusmi Tea – Chinese Green Tea (Kusmi), OnkoTea® – Base Tea Blend (Onko), and Lipton Tea – Lipton Black Tea (Lipton).

    Panels of breast cancer cells, melanoma cells, liver cancer cells, and bladder cancer cells were tested. The results showed that:

    1. The effects of tea on cell proliferation was concentration dependent. At lower concentrations (< 100 µg/mL), all the 4 tea extracts increased cell viability at 24h treatment. The increase of cell viability diminished, and inhibitory effects was observed as the treatment duration was longer. At a higher concentration range (200 – 800 µg/mL), all 4 tea products inhibited the cancer cells, typically with OnkoTea® showing the best inhibitory effect and Lipton tea the least.
    2. The inhibitory effect was cell line dependent. The melanoma cells A375 cells showed the best sensitivity to the OnkoTea® treatment, which harbors homologous BRAF mutation. The bladder cancer cell line 5637 showed good sensitivity to the tea products, whereas the liver cancer cell line HepG2 was the most resistant of all the tested cells.

    Volume 14 Issue 12 December 2019
    ©All Rights reserved by Ping Chen and Qi Chen

    Click hyperlink to access the full publication:
    Effects of Different Tea Products on the Growth of Cancer Cells

    If you have questions about our cancer research or would like to speak to a member of The Beljanski Foundation to learn more, please email or call us at 646-808-5583.

    Publication on Pao pereira Reducing BPH

    December 2019 – “Pao Pereira Extract Attenuates Testosterone-Induced Benign Prostatic Hyperplasia in Rats by inhibiting 5α-Reductase”

    by Jiakuan Liu, Tian Fang, Meiqian Li, Yuting Song, Junzun Li, Zesheng Xue, Jiaxuan Li, Dandan Bu, Wei Liu, Qinghe Zeng, Yidan Zhang, Shifeng Yun, Ruimin Huang & Jun Yan
    Scientific Reports 9, 19703 (2019). Doi: 10.1038/s41598-019-56145-z. E pub Dec 23 2019


    BPH (Benign Prostatic Hyperplasia) is so common and discussion about its causes is so frequent that we could well expect there to be good options for shrinking prostates back to normal size and restoring normal urination and prostate health. The usual options for BPH treatment are several widely prescribed drugs that work to reduce prostate size but are also associated with a variety of unpleasant side effects. New research shows that the Pao pereira plant extract, famous for its broad-spectrum anti-cancer effect, is an excellent BPH treatment — as good as the drugs — and it doesn’t induce negative side effects. The study was conducted at Nanjing University under the direction of Dr. Jun Yan.

    The key is a newly discovered modulating effect of the Pao extract on enzymatic pathways that promote the development of BPH. The new paper is entitled “Pao Pereira Extract Attenuates Testosterone-Induced Benign Prostatic Hyperplasia in Rats by Regulating 5a-reductase”. The critical enzyme pathway is 5a-reductase which converts testosterone to dihydrotestosterone (DHT)—a more active hormone that triggers the proliferation of prostate cells that leads to BPH. By lowering the level of 5alpha-reductase the Pao extract lowers the level of DHT thus removing the trigger for BPH.

    The study confirms that the Pao extract suppresses inflammation of the prostate by affecting the hormone imbalance that occurs in older men as testosterone levels drop and 5a-reductase increases the level of DHT. The extract is also effective at halting the overgrowth of BPH cells in the prostate by inducing cell cycle arrest. It also lowers the level of androgen receptor that mediates hormonal induction of BPH and reduces the level of PSA, the well-known marker for prostate inflammation and cancer. And referring to cancer, by lowering prostate inflammation Pao pereira extract lowers the risk of progression to prostate cancer.

    The experiments were conducted both in vitro and in vivo and no evidence for side effects were seen. The data indicates that the Pao pereira extract provides an ideal therapeutic response for BPH that can be taken without concern for negative side effects. This new work, together with previous research, shows that Pao pereira is effective against all stages of prostate health problems including chronic inflammation, prostate cancer and advanced prostate cancer.


    Scientific Reports Pao Pereira Reduces BPH

    Benign prostatic hyperplasia (BPH) is one of the most common diseases in the urinary system of elderly men. Pao extract is an herbal preparation of the bark of the Amazon rainforest tree Pao Pereira (Geissospermum vellosii), which was reported to inhibit prostate cancer cell proliferation. Herein we investigated the therapeutic potential of Pao extract against BPH development in a testosterone-induced BPH rat model. The administration of testosterone induced the prostate enlargement, compared with the sham operated group with vehicle treatment. The BPH/Pao group showed reduced prostate weight comparable with BPH/finasteride group. Notably, Pao treatment did not significantly reduce body weights and sperm number of rats, compared with the control group. Furthermore, Pao extract treatment reduced the proliferative index in prostate glands and testosterone-induced expression levels of AR, as well as androgen-associated proteins such as SRD5A1 and PSA. Moreover, Pao extract and its active component, flavopereirine, induced cytotoxicity on human prostate epithelial RWPE-1 cells in a dose- and time- dependent manner with G2/M arrest. Consistently, Pao extract and flavopereirine suppressed the expression levels of SRD5A1, AR and PSA, respectively. Together, these data demonstrated that Pao extract suppresses testosterone-induced BPH development through inhibiting AR activity and expression, and suggested that Pao extract may be a promising and relative safe agent for BPH Treatment.

    BPH Treatment & Pao Pereira Data

    BPH Treatment Pao Pereria

    The figure above shows the effects of Pao extract on rat prostate. (a) Schematic presentation of experimental procedure. Sham group as control group: After sham operation, rats were treated with i.p. injection of corn oil and oral saline; BPH/Veh group: After castration, rats were i.p. injection of 5 mg/kg testosterone propionate (TP) daily and were intragastric administrated with saline; BPH/FN (Finasteride) and BPH/Pao groups: After castration, rats were i.p. injection of 5 mg/kg TP and intragastic administration of finasteride (10 mg/kg) or Pao extract (20 mg/kg) daily for 28 days, respectively. (b) The representative photos of the dissected prostate glands from four groups. (c) The weight of whole prostate without urethra. (d) The changes in the rat prostate index of four groups. (e) Effect of Pao extract on body weight. n = 5; **p < 0.01; ***p < 0.001.

    BPH Treatment Pao Pereria Figure 2

    The figure above shows the histopathological analysis of the prostate tissues in the testosterone-induced BPH rats after being treated with Pao extract. (a) H&E staining of the sham control, BPH/Veh, BPH/FN and BPH/Pao groups. The sections were photographed by microscope. The scale bars in photos above are 50 μm and in photos below are 20 μm. (b) Quantification of the thickness of epithelial layers; (c) quantification of the fold changes of the lumen areas among four groups. ***p < 0.001.

    Download Pao Pereira Extract Attenuates Testosterone-Induced Benign Prostatic Hyperplasia in Rats by inhibiting 5α-Reductase

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      Milestone in Cancer Stem Cell Research

      Pao Pereira Fights Cancer Stem Cells!

      New research just published in Integrative Cancer Therapies shows that the Pao pereira plant extract inhibits pancreatic cancer stem cells. This dramatic result means that the extract will be useful for preventing cancer recurrence.

      Everyone knows that cancer treatments are not cancer cures. Chemotherapy treatments kill cancer cells and shrink tumors, but the cancer often comes back. Cancer stem cells (CSCs) are the culprit because they are resistant to anti-cancer drugs, they can metastasize and form new tumors. CSCs make up a small fraction of the cells in a tumor that survive after the majority of primary cancer cells are killed by anti-cancer drugs. The CSCs persist, spread and restart tumor growth.

      CSCs are also referred to as cancer stem-like cells because they share the capacity for self-proliferation with normal stem cells that are involved in the renewal and repair of tissues, but unlike normal stem cells, CSCs are cancerous and drug resistant.

      In vitro experiments show Pao pereira reducing the number of pancreatic CSCs (see figure below).

      As the amount of Pao pereira is increased, the number of pancreatic cancer stem cells (spheroids) decrease dramatically. In additional cell-based studies, the Pao pereira extract also specifically reduced the pancreatic cancer stem cell population.

      In vivo experiments showed that Pao pereira inhibited the capacity of pancreatic CSCs to form tumors – the defining characteristic of CSCs – and significantly reduce the volume of tumors that did form (see figure below).

      At day 6 (Figure A), tumor formation rate in the control group reached 80%, while in Pao-treated group it was only 10%. At day 20, when the treatment stopped, all mice in control group were bearing tumors on both flanks (100% tumor formation), while the Pao-treated group only had 30% tumor formation. Growth of the formed tumors was also inhibited by Pao pereira treatment compared with the control group (Figure B). A long-term inhibitory effect in tumor growth was observed after treatment had stopped. Pao pereira works by lowering the signals that trigger the pancreatic cancer stem-like cell properties that lead to cancer recurrence.

      The researchers at Kansas University Medical Center concluded that Pao pereira holds great promise as a novel treatment for targeting pancreatic CSCs, an essential component of treatments that will lead to real cancer cures.

      Extract of the Medicinal Plant Pao Pereira Inhibits Pancreatic Cancer Stem-Like Cell In Vitro and In Vivo

      Rauwolfia Vomitoria Effective Against Pancreatic Cancer Stem Cells

      May 2018 – “Inhibition of pancreatic cancer stem cells by Rauwolfia vomitoria extract”

      by Ruochen Dong, Ping Chen and Qi Ch
      ONCOLOGY REPORTS 40: 3144-3154, 2018.

      Rauwolfia Vomitoria Active Against Cancer Stem Cells

      In vitro experiments show Rauwolfia vomitoria reducing the number of pancreatic CSCs in a variety of cell lines (see figure below).

      Cancer Stem Cells and Rauwolfia Vomitoria in vitro

      As the amount of Rauwolfia vomitoria is increased, the number of pancreatic cancer stem cells drastically decreases.

      Even more impressive, the in vivo data demonstrates that Rauwolfia counteracts the defining behavior of CSCs, their ability to promote formation and growth of new pancreatic tumors. The charts below show how the single Rauwolfia treatment significantly inhibited the tumor formation rate (C) and the size of formed tumors (D).

      Cancer Stem Cells and Rauwolfia Vomitoria in vivo

      The researchers at Kansas University Medical Center concluded that, much like Pao pereria, Rauwolfia vomitoria also holds great promise as a novel treatment for targeting pancreatic CSCs. The two natural extracts are a vital method of treatments that are bringing new, real cancer cures.

      Click below read the full publication:
      Inhibition of pancreatic cancer stem cells by Rauwolfia vomitoria extract

      All Green Teas are Not Equal in Anti-Cancer Effect

      All Green Teas are Not Equal in Anti-Cancer Effect:  Research shows that a special green tea blend is more effective against breast cancer.



      The results of a new study comparing the anti-cancer effects of several well-known teas has shown that a newly developed blend of green teas was superior at inhibiting the growth of two breast cancer cell lines, including one highly metastatic cell line. This blend combines four different green teas: Ceylon Green, Bi Lou Chun, Organic Gunpowder and Dragonwell.

      Blend of teas selected by Mirko Beljanski

      The anti-cancer potential of each of these teas was originally identified by Prof. M. Beljanski of the Pasteur Institute using the Oncotest, an assay he developed for detecting carcinogenic and anti-carcinogenic compounds. The new study confirms the activity of a combination of the four green teas against breast cancer cells.

      Green teas have long been associated with anti-oxidant and anti-cancer benefits, but the new research shows that the activity of this specific combination stands out. When tested against other teas including Bigelow®, Kusmi®, and Lipton®, the four green tea blend had the most potent anti-cancer effect on the cultured breast cancer cells. Data in the figure below show the viability of the breast cancer cell lines after exposure to extracts of each of the teas tested (400 micrograms/ml). The green tea blend (OnkoTeaTM) was the best at reducing the number of breast cancer cells.

      Onkotea and breast cancer

      This research was conducted at a prominent medical center that has conducted many successful projects on behalf of The Beljanski Foundation.  Further research is planned to explore the effects of the green tea blend on other types of cancer.

      Publication on the anti-cancer and anti-inflammatory activities of Pao pereira

      November 2013 – “Pao pereira Extract Suppresses Castration- Resistant Prostate Cancer Cell Growth, Survival and Invasion Through Inhibition of NFkB Signaling”

      by Cunjie Chang, BS, Wei Zhao, MS, Bingxian Xie, BS, Yongming Deng, BS, Tao Han, BM, Yangyan Cui, BS, Yundong Dai, BS, Zhen Zhang, BS, Jimin Gao, MD, PhD, Hongqian Guo, MD, PhD, and Jun Yan, PhD.
      Integr Cancer Ther. 2014 May;13(3):249-58. Doi: 10.1177/1534735413510557. Epub 2013 Nov 27

      Nanjing_UniversityDr. Jun Yan from Dr. Aaron Katz’s laboratory at Columbia University Medical Center is continuing research (University of Nanjing, China) on the anti-cancer and anti-inflammatory activities of Pao Pereira (extract provided by Natural Source International, Ltd.).

      Dr. Jun Yan uses up to date techniques to determine how the Pao pereira extract exerts its effects, what molecules it influences in the cell and what responses are triggered. The publication shows that this Pao pereira extract is effective against prostate cancer cells that no longer respond to agents that interfere with hormone action.

      Such drugs are specifically designed to limit the effects of testosterone, the male hormone that stimulates the growth of prostate cancer cells. Over time, prostate tumors become resistant to these anti-hormones indicting that the cancer has become more aggressive. The study shows that these hormone independent prostate cancer cells are susceptible to the growth inhibition of Pao pereira as are prostate cancers that still respond to testosterone. The study also revealed that Pao pereira acts on a signaling complex called NFkB—a control center regulating cell survival, proliferation, and invasion. The results suggest that Pao pereira will be useful for men with advanced hormone-independent prostate cancer.

      Abstractrauwolfia and ovarian cancer

      Pao extract, derived from bark of Amazonian tree Pao Pereira, is commonly used in South American medicine. A recent study showed that Pao extract repressed androgen-dependent LNCaP prostate cancer cell growth. We hypothesize that Pao extract asserts its anticancer effects on metastatic castration-resistant prostate cancer (CRPC) cells. Pao extract suppressed CRPC PC3 cell growth in a dose- and time-dependent manner, through induction of apoptosis and cell cycle arrest. Pao extract treatment induced cell cycle inhibitors, p21 and p27, and repressed PCNA, Cyclin A and Cyclin D1. Furthermore, Pao extract also induced the upregulation of pro-apoptotic Bax, reduction of anti-apoptotic Bcl-2, Bcl-xL , and XIAP expression, which were associated with the cleavage of PARP protein. Moreover, Pao extract treatment blocked PC3 cell migration and invasion. Mechanistically, Pao extract suppressed phosphorylation levels of AKT and NFκB/p65, NFκB DNA binding activity, and luciferase reporter activity. Pao inhibited TNFα-induced relocation of NFκB/p65 to the nucleus, NFκB/p65 transcription activity, and MMP9 activity as shown by zymography. Consistently, NFκB/p65 downstream targets involved in proliferation (Cyclin D1), survival (Bcl-2, Bcl-xL , and XIAP), and metastasis (VEGFa, MMP9, and GROα/CXCL1) were also downregulated by Pao extract. Finally, forced expression of NFκB/p65 reversed the growth inhibitory effect of Pao extract. Overall, Pao extract induced cell growth arrest, apoptosis, partially through inhibiting NFκB activation in prostate cancer cells. These data suggest that Pao extract may be beneficial for protection against CRPC.

      Pao pereira Extract Suppresses Castration- Resistant Prostate Cancer Cell Growth, Survival and Invasion Through Inhibition of NFkB Signaling”

      Clinical trial with Beljanski’s RNA Fragments Beljanski

      October 2010 – “Dose Escalation Study of an Antithrombacytopenic Agent in Patients with Chemotherapy Induced Thrombocytopenia”

      by Robert D Levin, MaryAnn Daehler, James F Grutsch, John L Hall, Digant Gupta, Christopher G Lis. Levin
      Online magazine Bio Med Central BMC Cancer 10:565 – 2010.

      Low platelet counts, a condition called Thrombocytopenia, is a side effect of chemo drugs that damage the bone marrow stem cells that normally produce platelets. The Phase I trial showed that Beljanski’s RNA fragments could prevent thrombocytopenia by inducing the production of new platelets. Patients taking the RNA fragments had their platelet levels return to normal and chemotherapy treatments were completed without dose reductions, platelet transfusions, or suspensions. The RNA fragments protected platelet levels in patients with many different types of cancer who were taking many different anti-cancer drugs. Moreover, patients did not suffer any negative side effects as a result of taking the RNA fragments. The results suggest further studies aimed at establishing the RNA fragments as a standard component in all chemotherapies that cause significant platelet loss.

      CTCA - RNA FragmentsIn 2004, the Prestigious Cancer Treatment Centers of America® (CTCA) completed a clinical trial on the Beljanski’s formula of RNA fragments, which was conducted among cancer patients undergoing different chemotherapies. Thanks to the RNA fragments prepared according to Dr. Beljanski’s proprietary process, the cancer patients all recovered a stronger immune system and managed to go through the end of their chemotherapy treatment without getting thrombocytopenia – a frequent and dangerous side effect of the chemotherapy drugs.


      Background: Preclinical studies demonstrated that small chain RNA fragments accelerate the recovery of platelets numbers in animals exposed to high doses of chemotherapeutic drugs. There is anecdotal data supporting the same application in humans. The Phase I clinical trial described here was designed to investigate the relationship between the administration of small chain RNA fragments and the recovery in platelets following Chemotherapy Induced Thrombocytopenia (CIT).

      Methods: Cancer patients with solid tumors that experienced post chemotherapy thrombocytopenia with a nadir of < = 80,000 platelets/ml were eligible for this clinical trial. There were no exclusions based on ECOG status, tumor type, tumor burden or chemotherapeutic agents. Patients received a unique preparation of RNA derived from either E. coli or yeast. Ten patients per group received 20, 40, or 60 mg as a starting dose. Subjects selfadministered RNA fragments sublingually on an every other day schedule while undergoing chemotherapy. The dose was escalated in 20 mg increments to a maximum dose of 80 mg if the nadir was < 80,000 platelets/ml at the start of the next cycle. Subjects were treated for three cycles of chemotherapy with the maximum effective dose of RNA fragments. Subjects continued on planned chemotherapy as indicated by tumor burden without RNA fragment support after the third cycle. Subjects kept a diary indicating RNA fragment and magnesium administration, and any experienced side effects.

      Results: Patients receiving E. coli RNA fragments demonstrated a more rapid recovery in platelet count and higher nadir platelet count. None of the patients receiving the E. coli RNA fragments required a chemotherapy dose reduction due to thrombocytopenia. The optimal dose for minimizing CIT was 80 mg. Conversely, subjects receiving yeast RNA fragments with dose escalation to 80 mg required a chemotherapy dose reduction per American Society of Clinical Oncology guidelines for grade 3 and 4 thrombocytopenia.

      Conclusions: Patients receiving myelosuppressive chemotherapy experienced an improvement in the platelet nadir and shorter recovery time when receiving concurrent E coli RNA fragments, when compared to patients who received yeast RNA fragments. These data indicate that 60 and 80 mg doses of E. coli RNA accelerated platelet recovery. Further clinical investigations are planned to quantify the clinical benefits of the E. coli RNA at the 80 mg dose in patients with chemotherapy induced thrombocytopenia.

      Trial Registration: Clinical Identifier: NCT01163110

      Dose Escalation Study of an Antithrombacytopenic Agent in Patients with Chemotherapy Induced Thrombocytopenia

      Publication on Rauwolfia Vomitoria Extract against Pancreatic Cancer

      March 2014 – “Antitumor Activities of Rauwolfia vomitoria Extract and Potentiation of Gemcitabine Effects Against Pancreatic Cancer”

      by Jun Yu, PhD and Qi Chen, PhD
      Integrative Cancer Therapy. 2014 Apr 24;13(3):217-225.

      Kansas universityThe fourth publication from the scientific collaboration between the Beljanski Foundation and Kansas University Medical Center has been released. It shows, in vitro and in vivo, the effectiveness of Rauwolfia vomitoria (the extract prepared according to Dr. Mirko Beljanski’s proprietary process) on pancreatic cancer cells. This publication also confirms – if confirmation were needed – the non-toxicity of Rauwolfia (“Rau”), on healthy cells, its selective effect against cancer cells, and its excellent synergistic action with Gemcitabine chemotherapy. This study, conducted with extracts provided by Natural Source International, Ltd. is now available on The Beljanski Foundation website.

      For the purpose of this study, pancreatic cancer cells were inoculated into four groups of mice (control group treated with saline solution, mice treated with Gemcitabine alone, mice treated with Rauwolfia alone, and mice with combination treatment of Gemcitabine and Rauwolfia). As the tumors did not respond to Gemcitabine, Rauwolfia at either 20 mg/kg or 50 mg/kg provided observable inhibition in tumor growth. The combination of Gemcitabine + Rauwolfia at both doses also inhibited tumor progression. Notably, there were 2 mice in the Gemcitabine + Rauwolfia 50 mg/kg group that had complete tumor regression, an effect that was not observed in any other treatment group.

      Necropsy confirmed the imaging results at the end of the treatment. Gemcitabine at the dose used did not provide any reduction in tumor weight, when Rauwolfia alone decreased tumor weight by 53% at the daily dose of 20 mg/kg, and 46% at the daily dose of 50 mg/kg, compared with saline-treated control. By combining Rauwolfia with Gemcitabine, the decrease in tumor weight was 56% at both Rauwolfia doses. The improvement in tumor inhibition provided by the combination was significant compared with that of Gemcitabine alone.

      On assessing tumor metastasis, 12% of mice in the control group (saline-treated) and in the Rauwolfia 20 mg/kg group did not form metastasis, whereas all mice in Gemcitabine group formed metastasis. The percentage of metastasis free mice increased to 40% with both Rau 50 mg/kg treatment and Gemcitabine + Rauwolfia treatment, suggesting that Rauwolfia provided benefit in reducing metastatic potential while Gemcitabine did not.

      None of the mice demonstrated observable toxicity associated with the treatments. At the end of the experiments, major organs (kidney, liver, and spleen) were subjected to hematoxylin and eosin staining and histopathological analysis. No tissue damage was detected in the treatment groups, and there were no significant differences between the control group and the treated group. This data demonstrated that Rauwolfia at the dose used, either alone or combined with Gemcitabine, was effective and, at the same time, low in toxicity.
      This would open an avenue to reduce toxicity associated with chemotherapy.


      Pancreatic cancer is one of the most lethal malignancies with very limited treatment option. In the effort of enhancing the effect of the conventional chemotherapeutic drug gemcitabine against pancreatic cancer, we investigated in vitro and in vivo the anticancer effect of a β-carboline-enriched extract from the plant Rauwolfia vomitoria (Rau), either alone or in combination with gemcitabine, in preclinical pancreatic cancer models. Rau induced apoptosis in pancreatic cancer cells in a concentration-dependent manner, and completely inhibited colony formation of PANC-1 cells in soft agar.
      The combination of Rau and gemcitabine had synergistic effect in inhibiting cell growth with dose reduction effect for gemcitabine. In an orthotopic pancreatic cancer mouse model, PANC-1 tumor growth was significantly suppressed by Rau treatment. Metastasis was inhibited by Rau. Adding Rau to gemcitabine treatment reduced tumor burden and metastatic potential in the gemcitabine non-responsive tumor. These data suggest that Rau possesses anti–pancreatic cancer activity and could improve effect of gemcitabine.

      Antitumor Activities of Rauwolfia vomitoria Extract and Potentiation of Gemcitabine Effects Against Pancreatic Cancer

      Publication on Pao pereira Extract against Pancreatic Cancer

      March 2013 – “Inhibition of Pancreatic Cancer and Potentiation of Gemcitabine Effects by the Extract of Pao Pereira”

      Oncology Reports” Journal (doi: 10.3892/or.2013.2461)

      Kansas universityThe scientific team at the University of Kansas Medical Center has just published a new article entitled, “Inhibition of Pancreatic Cancer and Potentiation of Gemcitabine Effects by the Extract of Pao Pereira” in the “Oncology Reports” Journal (doi: 10.3892/or.2013.2461).

      This scientific paper describes the results of in vitro and in vivo studies demonstrating the anti-cancer effect of the Pao pereira extract, used alone and in combination with gemcitabine (chemotherapy used in most cancer treatments), in multiple cancer cell lines including those of the pancreas resistant to anti-mitotic drugs.

      Pre-clinical studies conducted with mice, both in vitro an in vivo, confirm that adding Pao Pereira to the treatment regiment reduced the concentration of Gemcitabine to produce an equitoxic effect on pancreatic cancer cells. The Pao’s anti-cancer effect was seen in vivo and in vitro working alone and in conjunction with other chemotherapy treatments. Over the course of the study, the Pao pereira was seen to suppress tumor growth significantly, by up to 72%.

      This confirms another study, published just a few years ago, out of Columbia University demonstrating that the two plant extracts, Pao Pereira and Rauwolfia Vomitoria, contain Beta-Carbolines with effective anti-cancer properties which inhibit cancer cell growth, even in those cells resistant to drugs used in chemotherapy. This study, specific to anti-prostate cancer activity, also substantiated that all healthy cells were not negatively affected, thus demonstrating the safety of the plant extracts prepared by the Beljanski method.

      With such promising initial results, the research will continue on Pao Pereira to assess its effectiveness on other types of cancerous cells!


      Kansas - Pao - PancreasLack of effective therapy is a major problem in the treatment of pancreatic cancer. In the present study, we investigated a natural product, the extract of Pao Pereira (Pao), for its anti-pancreatic cancer effect in vitro and in vivo, either alone or in combination with the first-line chemotherapeutic drug gemcitabine (Gem). Pao induced dose-dependent apoptosis to all five tested pancreatic cancer cell lines. The combination of Pao and Gem had a synergistic effect in the inhibition of cell growth, with combination indices (CIs)

      Inhibition of Pancreatic Cancer and Potentiation of Gemcitabine Effects by the Extract of Pao Pereira

      Clinical Trial on the synergistic effects of Rauwolfia vomitoria and Pao pereira on elevated PSA

      April-May 2010 – “Two Herbal Extracts for Protecting Prostate Cell DNA – IMCJ April 2010”

      by Melissa Burchill, RN, CDN – IMCJ (Integrative Medicine: A Clinician’s Journal)

      A clinical trial began in 2006 and enrolled some 42 patients with elevated prostate specific antigen (PSA) readings (averaging 8 to 10 on the PSA scale) and a negative biopsy a group of men that in the industrialized world numbers in the millions.

      One of the primary goals of the clinical trial was to determine if the plant extracts were safe. The research team did a dose escalation trial. The trial started at two capsules but has gone much higher, and so far all doses tested have been without side effects.

      “We now know that this combination of Beljanski’s extracts can significantly lower PSAs in a 12-month period. Also we have had very few patients convert to prostate cancer and have found a number of patients who have had a dramatic improvement in their urinary symptoms. Men are clearly having less frequency, better streams and better flow rates. They are not getting up at night as often.

      “The bottom line is that it appears our early results are reason to be very encouraged by Beljanski’s extracts’ ability to lower PSA and help older men urinate better, too.”

      So how important are Beljanski’s findings to men’s health? “There are a lot of men undergoing PSA screening,” Dr. Katz said. “The PSA supposedly stands for “prostate specific antigen” but I say it is more accurately “patient stimulated anxiety.” When a man’s PSA is elevated, there could be many reasons for this, having nothing to do with cancer. But what we know now is that these cells that are growing can develop into cancer, and we would like to stop them from doing so. Also if the cells keep growing even in benign fashion, they will grow around the urethra and push in on it and provoke urinary symptoms in men. That’s where we want to lower the growth and division of prostate cells and that’s what we think we have shown with the extracts.

      Source: “The Columbia Connection” by L.Stephen Coles, MD., Ph.D. The Doctors’ prescription for healthy living


      Two Herbal extract prostateDuring his 50 years of research, biochemist and molecular biologist Mirko Beljanski, PhD, discovered 2 plant extracts that appeared to inhibit the growth of cancer cells without causing any harmful side effects. The research on these products and the preliminary indications from an ongoing clinical trial are the subject of this article. In summary, Dr Beljanski made great contributions to our understanding of basic life processes and cancer. He determined that, quite apart from the occurrence of genetic mutations of DNA, carcinogens can bind to and damage the DNA double helix, thus creating a destabilized and dysfunctional structure. Dr Beljanski associated this destabilization of the DNA with excess replication, aberrant gene expression, and increased cell multiplication, which are processes that may ultimately lead to cancer. To determine which substances cause DNA destabilization and thus can be considered carcinogenic, Dr Beljanski developed what he called the Oncotest as a way to determine the effect of a compound on the structure of DNA; compounds that enhanced either UV absorption or the level of in vitro DNA synthesis were considered to have carcinogenic properties. Through use of the Oncotest, Dr Beljanski also discovered 2 natural molecules from the tropical plants Pao pereira and Rauwolfia vomitoria that specifically recognized and bound to the damaged double helix, thus preventing the process of cell division. In vitro, these 2 natural extracts have been shown to inhibit the proliferation of a wide variety of cancer cells without affecting healthy cells. Experiments with animals confirmed these results, and preliminary work with humans has provided similar indications. Clinical studies using a combination of the pao and rauwolfia extracts have yielded encouraging preliminary results by reducing prostate-specific antigen levels in men and improving symptoms of benign prostatic hyperplasia.

      Two Herbal Extracts for Protecting Prostate Cell DNA – IMCJ April 2010